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qscript cdna synthesis kit  (Quanta Biosciences)


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    Structured Review

    Quanta Biosciences qscript cdna synthesis kit
    Qscript Cdna Synthesis Kit, supplied by Quanta Biosciences, used in various techniques. Bioz Stars score: 97/100, based on 3866 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/qscript+kits/qScript+cDNA+Synthesis+Kit/custom%4095047-025%4042436854
    Average 97 stars, based on 3866 article reviews
    qscript cdna synthesis kit - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Dissociation of TRIF bias and adjuvanticity
    Article Snippet: .. Human THP-1 monocytes (ATCC ® TIB-202TM) were cultured in 10% FBS/RPMI complete plus 10mM HEPES buffer, 1mM sodium pyruvate, and 50 μM β-ME; differentiated into macrophages using 100 nM PMA for 48 h, and “rested” for an additional 24 h before stimulation with TLR4 agonists, as previously described [ 47 ]. qRT-PCR Macrophages were harvested in Tripure reagent (Roche) at the indicated time points after stimulation and frozen at −80 o C. RNA was extracted by the manufacturer’s protocol and cDNA reverse synthesized from 1 μg RNA per sample using QScript kits (QuantaBio), as previously described [ 45 ]. qRT-PCR was performed on a 7900HT instrument (Applied Biosystems) using Power SYBR Green PCR master mix (Applied Biosystems) with primers for Hprt , Tnf, Il10, Il1b, Cxcl1, Ifnb1, Cxcl10 , and Ccl5 (Sigma, custom synthesis based on published sequences [ 48 ], confirmed in primer BLAST). ..

    Quantitative RT-PCR:

    Article Title: Dissociation of TRIF bias and adjuvanticity
    Article Snippet: .. Human THP-1 monocytes (ATCC ® TIB-202TM) were cultured in 10% FBS/RPMI complete plus 10mM HEPES buffer, 1mM sodium pyruvate, and 50 μM β-ME; differentiated into macrophages using 100 nM PMA for 48 h, and “rested” for an additional 24 h before stimulation with TLR4 agonists, as previously described [ 47 ]. qRT-PCR Macrophages were harvested in Tripure reagent (Roche) at the indicated time points after stimulation and frozen at −80 o C. RNA was extracted by the manufacturer’s protocol and cDNA reverse synthesized from 1 μg RNA per sample using QScript kits (QuantaBio), as previously described [ 45 ]. qRT-PCR was performed on a 7900HT instrument (Applied Biosystems) using Power SYBR Green PCR master mix (Applied Biosystems) with primers for Hprt , Tnf, Il10, Il1b, Cxcl1, Ifnb1, Cxcl10 , and Ccl5 (Sigma, custom synthesis based on published sequences [ 48 ], confirmed in primer BLAST). ..

    Article Title: Dissociation of TRIF bias and adjuvanticity
    Article Snippet: Human THP-1 monocytes (ATCC ® TIB-202TM) were cultured in 10% FBS/RPMI complete plus 10mM HEPES buffer, 1mM sodium pyruvate, and 50 μM β-ME; differentiated into macrophages using 100 nM PMA for 48 h, and “rested” for an additional 24 h before stimulation with TLR4 agonists, as previously described [ 47 ]. .. Macrophages were harvested in Tripure reagent (Roche) at the indicated time points after stimulation and frozen at −80 o C. RNA was extracted by the manufacturer’s protocol and cDNA reverse synthesized from 1 μg RNA per sample using QScript kits (QuantaBio), as previously described [ 45 ]. qRT-PCR was performed on a 7900HT instrument (Applied Biosystems) using Power SYBR Green PCR master mix (Applied Biosystems) with primers for Hprt , Tnf, Il10, Il1b, Cxcl1, Ifnb1, Cxcl10 , and Ccl5 (Sigma, custom synthesis based on published sequences [ 48 ], confirmed in primer BLAST). ..

    Article Title: CD40 signaling augments IL-10 expression and the tolerogenicity of IL-10-induced regulatory dendritic cells
    Article Snippet: .. RNA was isolated from cells using RNeasy (QIAGEN, ON) kits, while cDNAs were synthesized using qScript kits (Quanta Biosciences, MD), according to the supplier’s specifications. qRT-PCR reactions were performed using PerfCTa SYBR Green FastMix (Quanta Biosciences) with the appropriate primers in a C1000 Touch (BioRad, Mississauga, ON) thermocycler. ..

    Synthesized:

    Article Title: Dissociation of TRIF bias and adjuvanticity
    Article Snippet: .. Human THP-1 monocytes (ATCC ® TIB-202TM) were cultured in 10% FBS/RPMI complete plus 10mM HEPES buffer, 1mM sodium pyruvate, and 50 μM β-ME; differentiated into macrophages using 100 nM PMA for 48 h, and “rested” for an additional 24 h before stimulation with TLR4 agonists, as previously described [ 47 ]. qRT-PCR Macrophages were harvested in Tripure reagent (Roche) at the indicated time points after stimulation and frozen at −80 o C. RNA was extracted by the manufacturer’s protocol and cDNA reverse synthesized from 1 μg RNA per sample using QScript kits (QuantaBio), as previously described [ 45 ]. qRT-PCR was performed on a 7900HT instrument (Applied Biosystems) using Power SYBR Green PCR master mix (Applied Biosystems) with primers for Hprt , Tnf, Il10, Il1b, Cxcl1, Ifnb1, Cxcl10 , and Ccl5 (Sigma, custom synthesis based on published sequences [ 48 ], confirmed in primer BLAST). ..

    Article Title: Dissociation of TRIF bias and adjuvanticity
    Article Snippet: Human THP-1 monocytes (ATCC ® TIB-202TM) were cultured in 10% FBS/RPMI complete plus 10mM HEPES buffer, 1mM sodium pyruvate, and 50 μM β-ME; differentiated into macrophages using 100 nM PMA for 48 h, and “rested” for an additional 24 h before stimulation with TLR4 agonists, as previously described [ 47 ]. .. Macrophages were harvested in Tripure reagent (Roche) at the indicated time points after stimulation and frozen at −80 o C. RNA was extracted by the manufacturer’s protocol and cDNA reverse synthesized from 1 μg RNA per sample using QScript kits (QuantaBio), as previously described [ 45 ]. qRT-PCR was performed on a 7900HT instrument (Applied Biosystems) using Power SYBR Green PCR master mix (Applied Biosystems) with primers for Hprt , Tnf, Il10, Il1b, Cxcl1, Ifnb1, Cxcl10 , and Ccl5 (Sigma, custom synthesis based on published sequences [ 48 ], confirmed in primer BLAST). ..

    Article Title: Therapeutic reversal of food allergen sensitivity by mature retinoic acid-differentiated dendritic cell induction of LAG3 + CD49b - Foxp3 - regulatory T cells.
    Article Snippet: .. RNA was isolated from cells by using RNeasy kits (Qiagen, Toronto, Ontario, Canada), whereas cDNAs were synthesized with qScript kits (Quanta Biosciences, Gaithersburg, Md), according to the manufacturer’s specifications. .. Quantitative RT-PCR (qRT-PCR) reactions were performed with a PerfeCTa SYBRGreen FastMix (Quanta Biosciences) with appropriate primers in a C1000 Touch (Bio-Rad Laboratories, Mississauga, Ontario, Canada) thermocycler.

    Article Title: CD40 signaling augments IL-10 expression and the tolerogenicity of IL-10-induced regulatory dendritic cells
    Article Snippet: .. RNA was isolated from cells using RNeasy (QIAGEN, ON) kits, while cDNAs were synthesized using qScript kits (Quanta Biosciences, MD), according to the supplier’s specifications. qRT-PCR reactions were performed using PerfCTa SYBR Green FastMix (Quanta Biosciences) with the appropriate primers in a C1000 Touch (BioRad, Mississauga, ON) thermocycler. ..

    SYBR Green Assay:

    Article Title: Dissociation of TRIF bias and adjuvanticity
    Article Snippet: .. Human THP-1 monocytes (ATCC ® TIB-202TM) were cultured in 10% FBS/RPMI complete plus 10mM HEPES buffer, 1mM sodium pyruvate, and 50 μM β-ME; differentiated into macrophages using 100 nM PMA for 48 h, and “rested” for an additional 24 h before stimulation with TLR4 agonists, as previously described [ 47 ]. qRT-PCR Macrophages were harvested in Tripure reagent (Roche) at the indicated time points after stimulation and frozen at −80 o C. RNA was extracted by the manufacturer’s protocol and cDNA reverse synthesized from 1 μg RNA per sample using QScript kits (QuantaBio), as previously described [ 45 ]. qRT-PCR was performed on a 7900HT instrument (Applied Biosystems) using Power SYBR Green PCR master mix (Applied Biosystems) with primers for Hprt , Tnf, Il10, Il1b, Cxcl1, Ifnb1, Cxcl10 , and Ccl5 (Sigma, custom synthesis based on published sequences [ 48 ], confirmed in primer BLAST). ..

    Article Title: Dissociation of TRIF bias and adjuvanticity
    Article Snippet: Human THP-1 monocytes (ATCC ® TIB-202TM) were cultured in 10% FBS/RPMI complete plus 10mM HEPES buffer, 1mM sodium pyruvate, and 50 μM β-ME; differentiated into macrophages using 100 nM PMA for 48 h, and “rested” for an additional 24 h before stimulation with TLR4 agonists, as previously described [ 47 ]. .. Macrophages were harvested in Tripure reagent (Roche) at the indicated time points after stimulation and frozen at −80 o C. RNA was extracted by the manufacturer’s protocol and cDNA reverse synthesized from 1 μg RNA per sample using QScript kits (QuantaBio), as previously described [ 45 ]. qRT-PCR was performed on a 7900HT instrument (Applied Biosystems) using Power SYBR Green PCR master mix (Applied Biosystems) with primers for Hprt , Tnf, Il10, Il1b, Cxcl1, Ifnb1, Cxcl10 , and Ccl5 (Sigma, custom synthesis based on published sequences [ 48 ], confirmed in primer BLAST). ..

    Article Title: CD40 signaling augments IL-10 expression and the tolerogenicity of IL-10-induced regulatory dendritic cells
    Article Snippet: .. RNA was isolated from cells using RNeasy (QIAGEN, ON) kits, while cDNAs were synthesized using qScript kits (Quanta Biosciences, MD), according to the supplier’s specifications. qRT-PCR reactions were performed using PerfCTa SYBR Green FastMix (Quanta Biosciences) with the appropriate primers in a C1000 Touch (BioRad, Mississauga, ON) thermocycler. ..

    Polymerase Chain Reaction:

    Article Title: Dissociation of TRIF bias and adjuvanticity
    Article Snippet: .. Human THP-1 monocytes (ATCC ® TIB-202TM) were cultured in 10% FBS/RPMI complete plus 10mM HEPES buffer, 1mM sodium pyruvate, and 50 μM β-ME; differentiated into macrophages using 100 nM PMA for 48 h, and “rested” for an additional 24 h before stimulation with TLR4 agonists, as previously described [ 47 ]. qRT-PCR Macrophages were harvested in Tripure reagent (Roche) at the indicated time points after stimulation and frozen at −80 o C. RNA was extracted by the manufacturer’s protocol and cDNA reverse synthesized from 1 μg RNA per sample using QScript kits (QuantaBio), as previously described [ 45 ]. qRT-PCR was performed on a 7900HT instrument (Applied Biosystems) using Power SYBR Green PCR master mix (Applied Biosystems) with primers for Hprt , Tnf, Il10, Il1b, Cxcl1, Ifnb1, Cxcl10 , and Ccl5 (Sigma, custom synthesis based on published sequences [ 48 ], confirmed in primer BLAST). ..

    Article Title: Dissociation of TRIF bias and adjuvanticity
    Article Snippet: Human THP-1 monocytes (ATCC ® TIB-202TM) were cultured in 10% FBS/RPMI complete plus 10mM HEPES buffer, 1mM sodium pyruvate, and 50 μM β-ME; differentiated into macrophages using 100 nM PMA for 48 h, and “rested” for an additional 24 h before stimulation with TLR4 agonists, as previously described [ 47 ]. .. Macrophages were harvested in Tripure reagent (Roche) at the indicated time points after stimulation and frozen at −80 o C. RNA was extracted by the manufacturer’s protocol and cDNA reverse synthesized from 1 μg RNA per sample using QScript kits (QuantaBio), as previously described [ 45 ]. qRT-PCR was performed on a 7900HT instrument (Applied Biosystems) using Power SYBR Green PCR master mix (Applied Biosystems) with primers for Hprt , Tnf, Il10, Il1b, Cxcl1, Ifnb1, Cxcl10 , and Ccl5 (Sigma, custom synthesis based on published sequences [ 48 ], confirmed in primer BLAST). ..

    Isolation:

    Article Title: Therapeutic reversal of food allergen sensitivity by mature retinoic acid-differentiated dendritic cell induction of LAG3 + CD49b - Foxp3 - regulatory T cells.
    Article Snippet: .. RNA was isolated from cells by using RNeasy kits (Qiagen, Toronto, Ontario, Canada), whereas cDNAs were synthesized with qScript kits (Quanta Biosciences, Gaithersburg, Md), according to the manufacturer’s specifications. .. Quantitative RT-PCR (qRT-PCR) reactions were performed with a PerfeCTa SYBRGreen FastMix (Quanta Biosciences) with appropriate primers in a C1000 Touch (Bio-Rad Laboratories, Mississauga, Ontario, Canada) thermocycler.

    Article Title: CD40 signaling augments IL-10 expression and the tolerogenicity of IL-10-induced regulatory dendritic cells
    Article Snippet: .. RNA was isolated from cells using RNeasy (QIAGEN, ON) kits, while cDNAs were synthesized using qScript kits (Quanta Biosciences, MD), according to the supplier’s specifications. qRT-PCR reactions were performed using PerfCTa SYBR Green FastMix (Quanta Biosciences) with the appropriate primers in a C1000 Touch (BioRad, Mississauga, ON) thermocycler. ..



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